Using the polymerase chain reaction coupled with denaturing gradient gel electrophoresis to investigate the association between bacterial translocation and systemic inflammatory response syndrome in predicted acute severe pancreatitis

doi:10.3748/wjg.v11.i45.7142

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Dec 7, 2005 (publication date) through Aug 25, 2024

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World Journal of Gastroenterology

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1007-9327

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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

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World J Gastroenterol. Dec 7, 2005; 11(45): 7142-7147
Published online Dec 7, 2005. doi: 10.3748/wjg.v11.i45.7142

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Figure 1 PCR profiles of total DNA recovered from human blood samples and of E coli DNA (control) obtained using primers BD1 and BD2 specific for bacterial 16S rRNA region. Positive control: Chromosomal DNA from E coli. Lanes 1-11: total DNA extracted from human blood samples.

Citation: Pearce CB, Zinkevich V, Beech I, Funjika V, Ruiz AG, Aladawi A, Duncan HD. Using the polymerase chain reaction coupled with denaturing gradient gel electrophoresis to investigate the association between bacterial translocation and systemic inflammatory response syndrome in predicted acute severe pancreatitis. World J Gastroenterol 2005; 11(45): 7142-7147
URL: https://www.wjgnet.com/1007-9327/full/v11/i45/7142.htm
DOI: https://dx.doi.org/10.3748/wjg.v11.i45.7142