Upregulation of 25-hydroxyvitamin D3-1α-hydroxylase by butyrate in Caco-2 cells

Figure 2 Time-dependent effect of butyrate on 1α-25(OH)₂D₃ expression. A: Caco-2 cells were grown for 36 h in serum-free medium in the absence (lane 1) or presence of butyrate (3 mmol/L) (lanes 2-4). 1α-25(OH)₂D₃ mRNA was analyzed by semiquantitative RT-PCR with the fluorescent dye PicoGreen. B: Caco-2 cells were treated for 72 h in the absence (lane 1) or presence of butyrate (3 mmol/L) (lanes 2-4) and then harvested for immunoblot analysis. The band at approximately 55 ku corresponds to 1α-25(OH)₂D₃ protein. Quantitation of protein was performed using a luminescent image scanner. ^aP<0,05 vs control; NS: non significant.