Upregulation of 25-hydroxyvitamin D3-1α-hydroxylase by butyrate in Caco-2 cells

Figure 1 Effects of 1α-25(OH)₂D₃, 25(OH)₂D₃ and butyrate on differentiation of Caco-2 cells as assessed by AP activity. Cells were treated for 48 h with a medium supplemented with 10^-6 mol/L 1α-25(OH)₂D₃, 10^-6 mol/L 25(OH)₂D₃, 3 mmol/L butyrate, or with one of the combinations of butyrate (3 mmol/L) and 1α-25(OH)₂D₃ (10^–6 mol/L) or butyrate (3 mmol/L) and 25(OH)₂D₃, (10^–6 mol/L). Values are expressed as milliunits of AP activity per milligram cellular protein. ^aP<0.05; ^bP<0.01; ^dP<0.001; NS: non significant.