Oral vaccination of mice against rodent malaria with recombinant Lactococcus lactis expressing MSP-119

Figure 2 Immunoblotting analysis of MSP-1₁₉ expressed in L. lactis. Protein samples were first analyzed on 12% SDS-polyacrylamide gel and then transferred on nitrocellulose membrane followed by immunostaining with antiserum prepared by infecting mouse with P. yoelii parasites. lane 1, protein markers stained by amido black; lane 2, positive control of fusion protein GST-MSP-1₁₉ purified from E. coli cell lysate expressing the fusion protein by pGEX-MSP-1₁₉; lane 3, total protein of L. lactis cells harboring plasmid pL2-PSGT. The arrows indicate the position of fusion protein GST-MSP-1₁₉ and MSP-1₁₉.