Bifunctional chimeric SuperCD suicide gene -YCD: YUPRT fusion is highly effective in a rat hepatoma model

Figure 2 SRB cytotoxicity assay with stable BCD, YCD, SuperCD expressing Morris hepatoma (MH) cell lines generated by retroviral transduction. Untransduced MH3924A cells (MH naïve) and stably transduced Morris hepatoma cell lines (MH BCD, MH YCD, MH SuperCD) were seeded on 24-well plates. After 4 d of 5-FC treatment, the growth inhibition was determined by SRB staining and measured at 550 nm. All values were referred to that of the untransduced control cells without the addition of 5-FC and are given as percentage of surviving cells (control: 100%). All experiments were carried out at least in quadruplicate. Viability values plotted represent fitted medians and 95%CI.