Copyright
©2005 Baishideng Publishing Group Inc.
World J Gastroenterol. Nov 28, 2005; 11(44): 6910-6919
Published online Nov 28, 2005. doi: 10.3748/wjg.v11.i44.6910
Published online Nov 28, 2005. doi: 10.3748/wjg.v11.i44.6910
Figure 1 Mode of action of SuperCD (YCD::YUPRT) fusion.
Panel A: Schematic representation of biological pathways resulting in cytotoxicity following cytosine deaminase/uracil phosphoribosyltransferase (UPRT)-mediated toxification of the prodrug 5-FC. 5-FC: 5-fluorocytosine; 5-FU: 5-fluorouracil; 5-FUR: 5-fluorouridine; 5-FUMP: 5-fluorouridine 5'-monophosphate; CD: cytosine deaminase; YUPRT: yeast uracil phosphoribosyltransferase. Panel B: Schematic representation of retroviral vectors used, in which suicide gene expression was placed under the retroviral 5' LTR. Panel C: Western blot detection of suicide gene expression in retrovirally generated stable SuperCD/YCD/BCD-expressing Morris hepatoma (MH) cell lines employing polyclonal rabbit antisera directed against BCD (lanes 1 and 2) and YCD (for detection of both SuperCD and YCD; lanes 3-5). Lane 1: MH naïve cells (negative control); lane 2: MH BCD cells, exhibiting a 48-ku band specific for BCD; lane 3: MH naïve cells (negative control); lane 4: MH YCD cells, exhibiting a 18-ku band specific for YCD; lane 5: MH SuperCD cells, exhibiting a 40-ku band specific for the YCD::YUPRT fusion, i.e. SuperCD. Numbers on the right hand side indicate the sizes of the expected proteins.
- Citation: Graepler F, Lemken ML, Wybranietz WA, Schmidt U, Smirnow I, Groß CD, Spiegel M, Schenk A, Graf H, Lauer UA, Vonthein R, Gregor M, Armeanu S, Bitzer M, Lauer UM. Bifunctional chimeric SuperCD suicide gene -YCD: YUPRT fusion is highly effective in a rat hepatoma model. World J Gastroenterol 2005; 11(44): 6910-6919
- URL: https://www.wjgnet.com/1007-9327/full/v11/i44/6910.htm
- DOI: https://dx.doi.org/10.3748/wjg.v11.i44.6910