Toll-like receptor 4 plays an anti-HBV role in a murine model of acute hepatitis B virus expression

Figure 5 HBV replication was reduced in C3H/HeJ mice after adoptive transfer of splenocytes from C3H/HeN mice. 5×10⁶ splenocytes from C3H/HeN or C3H/HeJ mice were injected into C3H/HeJ or C3H/HeN mice intravenously at the day before pHBV3.6 injection, respectively. (A) The HBsAg in sera was detected as indicated day by ELISA. ^aP<0.05. (B) The total RNA or cytoplasmic DNA of liver was isolated at day 3 and was further analyzed for HBV transcription or replication by Northern or Southern blot. The transfection efficiency was verified by co-injection of pEGFP-N1 and EGFP RNA confirmation. The number below each land of Northern or Southern blot was represented as the relative fold of expression comparing to C3H/HeN mice. N, C3H/HeN; J, C3H/HeJ; SP, splenocytes. (C) The HBV-DNA in sera was isolated as on the indicated day and detected by PCR method. The number below each land was represented as the relative fold of expression comparing to day 2 result of C3H/HeN mice. M, 100-bp DNA ladder; Neg, no template control; P, pHBV3.6 5 ng; N, C3H/HeN; J, C3H/HeJ; SP, splenocytes.