Toll-like receptor 4 plays an anti-HBV role in a murine model of acute hepatitis B virus expression

Figure 3 HBV-specific immune responses in the liver or spleen of C3H/HeN or C3H/HeJ mice after hydrodynamic-injection of pHBV3. 6. Groups of four C3H/HeN or C3H/HeJ mice were injected intravenously with 10 μg of pHBV3.6 by hydrodynamics-based transfection. The intrahepatic lymphocytes (A and B) or splenocytes (C and D) were isolated at d 8 post injection. After being co-cultured with HBsAg and HBcAg expressing syngenic MBT-2 cells (MBT-2-SC) for 48 h, the IFN-γ (A and C), TNF-α (B) or IL-12 (D) in the culture supernatant was analyzed by ELISA method. ^aP<0.05 vs naïve MBT-2-SC group.