Grapefruit-seed extract attenuates ethanol-and stress-induced gastric lesions via activation of prostaglandin, nitric oxide and sensory nerve pathways

Figure 6 Determination of SOD mRNA (left panel) by RT-PCR and by ratio of SOD mRNA over β-actin (right panel) in the vehicle-control intact gastric mucosa (lane 1) and in those treated with GSE given i. g. in graded doses of 16 mg/kg (lane 2), 32 mg/kg (lane 3) and 64 mg/kg (lane 4) prior to the exposure to ethanol, M-DNA size marker, NC-negative control. Mean±SE of 4–6 rats. ^aP < 0.05 vs vehicle (Veh), ^bP < 0.01 vs ethanol alone group. ^cP < 0.05 vs respective values in animals with capsaicin denervation. administration, ^cP < 0.05 vs respective values in animals treated with L-NNA. vs vehicle (Veh), ^bP < 0.01 vs respective values in animals without indomethacin or rofecoxib pretreatment.