Integration of hepatitis B virus DNA into chromosomal DNA during acute hepatitis B

Figure 2 Schematic representation of the HBV DNA integrant amplified from the serum of acute hepatitis patient (#0962) using primers 455 (+) and 1 800 (–) and cloned into pPCR-Script Amp SK (+) plasmid relative to the HBV genome. The lower part of the figure represents the genetic organization of the four open reading frames of HBV, preC/C: precore/core, P: polymerase gene, S: surface gene and X: X gene. Numbering according to nucleotide position of HBV GenBank accession no. V00866 where the EcoRI cleavage site is position 1.