Integration of hepatitis B virus DNA into chromosomal DNA during acute hepatitis B

Table 1 Oligonucleotide primers and PCR cycling profiles used in the study

Fragment	Primer	Position1	Sequence	Denaturation	Annealing	Extension	Size2
A	455(+)	455-474	5’-CAAGGTATGTTGCCCGTTTG-3				1 345
	1 800(–)	1 800-1 773	5’-AGACCAATTTATGCCTACAGCCTCCTA-3’	94 °C 30 s	62 °C 30 s	72 °C 90 s
B	1 687(+)	1 687-1 708	5’-CGACCGACCTTGAGGCATAC-3’
	685(-)	704-685	5’-CGAACCACTGAACAAATGGC-3’	94 °C 30 s	63 °C 30 s	72 °C 30 S	2 198

(+) sense (–) anti-sense.

¹Denotes the nucleotide position of HBV adw genome (GenBank accession no. V00866) where the EcoRI cleavage site is position 1². Size of the amplicons in base pairs.