Expression of matrix metalloproteinases and tissue inhibitor metalloproteinases increases in X-irradiated rat ileum despite the disappearance of CD8a T cells

Figure 2 Gelatinases activities in ilea after X-irradiation with a single dose of 10 Gy. A: Study of gelatinases activities by zymography. Lane STD shows MMP-2 and MMP-9 standards, lane 2-4, 5-7; 8-10; 11-13 shows gelatinase activities in ilea from control rats (C) and one (D1), three (D3) and seven (D7) days after X-irradiation respectively; B: Densitometric analysis of zymography. Results are mean±SE, significantly different from controls: ^aP<0.05, ^bP<0.01 vs others. C1-3: Analysis of MMP-2, MMP-9 and MMP-14 gene expression. mRNA levels were measured by real time RT-PCR in control ilea from control rats (C) or one (D1), three (D3) and seven (D7) days after X-irradiation respectively. Results are mean±SE, significantly different from controls: ^aP<0.05, ^bP<0.01 vs others.