Endothelial adhesion of synchronized gastric tumor cells changes during cell cycle transit and correlates with the expression level of CD44 splice variants

Figure 3 Facs analysis of CD44 and CD44 splice variant expression of MKN45 cells. Primary antibodies, indicated in methods, were conjugated with fluorescein isothiocyanate (FITC). CD44 surface expression of MKN45 cells was then measured using a FACscan (FL-1H (log) channel histogram analysis; 1 x104 cells/scan) and expressed as mean fluorescence units (MFU). Mouse IgG1-FITC was used as an isotype control. Mean±SD of n = 3 experiments.