Classifying genotype F of hepatitis B virus into F1 and F2 subtypes

Table 2 Primers used for PCR and sequencing

Primer1	Polarity	Sequence (5’–3’)	Positions (nt)
HBV/Ff1	Sense	GGG TCA CCA TAT TCT TGG GAA	2 814–2 834
HBV/Fr1	Antisense	CGT TGC CGA GCA ACG GGG TAA AGG	1 163–1 140
HBV/Ff2	Sense	GTT TCT CCT GGC TCA GTT TA	660–679
HBV/Fr2	Antisense	AAA AAG TTG CAT GGT GCT GG	1 825–1 806
HBV/Ff3	Sense	ACG TCG CAT GGA GAC CAC CG	1 601–1 620
HBV/Fr3	Antisense	GAA CTG GAG CCA CCA GCA GG	75–56
HBV/Ff4	Sense	CCT CCT GCT TCC ACC AAT CG	3 124–3 143
HBV/Fr4	Antisense	AGA TGA GGC ATA GCA GCA GGA TG	431–409
HBV/Ff5	Sense	GTC TAG ACT CGT GGT GGA CTT CTC	246–269
HBV/Fr5	Antisense	AAG CCA GAC AGT GGG GGA AAG C	730–709
HBV/Ff6	Sense	CTC GCC AAC TTA CAA GGC CTT T	1 098–1 119
HBV/Fr6	Antisense	GAT TCA GCG CCG ACG GGA CGT A	1 447–1 426
HBV/Ff7	Sense	CTC TGC CGA TCC ATA CTG CGG AA	1 256–1 278
HBV/Fr7	Antisense	TGA GAT CTT CTG CGA CGC GGC	2 431–2 410
HBV/Ff8	Sense	TCA GGC AAG CTA TTT TGT GCT GG	2 064–2 086
HBV/Fr8	Antisense	TGT TCA CAT TTG TGT ATC AAA T	2 587–2 565
HBV/Ff9	Sense	GCC GCG TCG CAG AAG ATC TCA A	2 410–2 431
HBV/Fr9	Antisense	AAA ATG AGG CGC TAT GTG TGG ATT	2 811–2 788

¹The first three pairs of primers (HBV/Ff1 to HBV/Fr3) were used for PCR and sequencing, while the remaining six pairs (HBV/Ff4 to HBV/Fr9) for sequencing only.