Purification and application of C-terminally truncated hepatitis C virus E1 proteins expressed in Escherichia coli

Figure 2 Expression and purification of E1 fragments in E. coli Samples analyzed on standard reductive SDS-PAGE followed by Coomassie Brilliant Blue staining. (A) and (B): expression and purification of E1Z₃₄₀ and E1Z₂₆₂W_326N. Lane 1: induced TG-1(pQE8) as negative control; lane 2: whole-cell sample after induction; lane 3: soluble fraction after sonication; lane 4: insoluble fraction after sonication; lane 5: purified products. (C): whole-cell samples of induced TG-1 carrying pQE8 (lane 1), pQE8/E1W (lane 2), pQE8/E1W₃₂₆ (lane 3), and pQE8/E1Z₂₆₂W, respectively.