Endothelin-1 stimulates contraction and migration of rat pancreatic stellate cells

Figure 5 ET-1 induced migration of PSCs. Cell migration was assessed using modified Boyden chambers with 8-mm pore filters. Serum-starved PSCs were left untreated ("Con" or were treated with ET-1 (at 100 nmol/L) in the lower chamber in the absence or presence of BQ-123 (×23×at 10 mmol/L), BQ-788 (×88×at 10 mmol/L), Y-27632 (at 25 mmol/L), or U0126 ("U"at 5 mmol/L). After 24-h incubation, the cells migrated to the underside of the filter were stained, and counted under a light microscopy. As positive controls, cells were treated with PDGF-BB (at 25 mg/L) in the lower chamber, and cell migration was assessed in a similar manner. Data are shown as mean±SD (% of the control, n = 6). bP<0.01 vs control (serum-free medium only), aP<0.05, dP<0.01 vs ET-1 treatment only.