Endothelin-1 stimulates contraction and migration of rat pancreatic stellate cells

Figure 4 ET-1 induced contraction of PSCs. PSCs were plated on the surface of collagen lattices prepared in each well of a six-well plate and incubated for 2 h to allow adherence. The collagen lattices were then detached from the plate by gentle circumferential dislodgment. Serum-starved PSCs were left untreated ("Con" or were treated with ET-1 (at 100 nmol/L) in the absence or presence of BQ-123 (×23×at 10 mmol/L), BQ-788 (×88×at 10 mmol/L), Y-27632 (at 25 mmol/L), or U0126 ("U"at 5 mmol/L). Lattices were then detached from the plate, and incubated in serum-free medium (control, "Con" with or without 100 nmol/L ET-1. After 24-h incubation, we measured the diameter of the collagen lattices, and calculated the × of the original diameter ("Orig"= 35 mm)× Data are shown as mean±SD (% of the original diameter, n = 8). bP<0.01 vs control (serum-free medium only), dP<0.01 vs ET-1 treatment only.