Endothelin-1 stimulates contraction and migration of rat pancreatic stellate cells

Figure 3 Y-27632 decreased stress fiber formation. A: PSCs were treated with ET-1 (at 100 nmol/L) in the presence of Y-27632 at the indicated concentrations (at mmol/L) for 30 min. Total cell lysates (approximately 100 mg) were prepared, and the levels of phosphorylated and total MLC were determined by Western blotting. In parallel experiments, Y-27632 was withdrawn after 30-min incubation, and replaced with 10% FBS. After 48-h incubation with 10% FBS, total cell lysates were prepared, and the levels of phosphorylated and total MLC were determined by Western blotting; B-D: PSCs were incubated with ET-1 (at 100 nmol/L) in the absence (B) or presence (C) of Y-27632 (at 25 mmol/L) for 24 h, and stained with rhodamine-labeled phalloidin. In parallel experiments, Y-27632 was withdrawn and replaced with 10% FBS. After 48-h incubation with 10% FBS, PSCs were stained with rhodamine-labeled phalloidin (D). Original magnification: ×0 objective.