Endothelin-1 stimulates contraction and migration of rat pancreatic stellate cells

Figure 1 Culture-activated PSCs expressed ET and ET receptors. A: Total RNA was prepared from culture-activated, serum-starved PSCs. The expression of preproET-1, ET_A receptor and ET_B receptor was examined by reverse transcription-PCR. The expected sizes of the PCR products were 382 bp for preproET-1, 321 bp for ET_A receptor, and 297 bp for ET_B receptor. sm: size marker (fX174 HaeIII digest). bp: bp; B-D: Serum-starved PSCs were grown directly on slides. Immunostaining for ET-1 (panel B), ET_A receptor (panel C), and ET_B receptor (panel D) was performed using a streptavidin-biotin peroxidase complex detection kit. Original magnification: ×0 objective.