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©The Author(s) 2005.
World J Gastroenterol. Oct 21, 2005; 11(39): 6159-6164
Published online Oct 21, 2005. doi: 10.3748/wjg.v11.i39.6159
Published online Oct 21, 2005. doi: 10.3748/wjg.v11.i39.6159
Table 1 Construction of recombinant plasmid and expression of engineering bacterium
| Engineering bacterium | Recombinant plasmid | Expression host (Escherichia colistrain) | Expression Vector used | Fusion Tag |
| E.coli BL-TB | pET32-RBD | BL21(DE3) | pET32a | TrxA·tag S·tag |
| E.coli R-TB | pET32-RBD | Rosetta-gamiB(DE3) | pET32a | TrxA·tag S·tag |
| E.coli BL-GB | pGEX-RBD | BL21(DE3) | pGEX-4t-2 | GST·tag |
| E.coli BL-MB | pET-MBP-RBD | BL21(DE3) | pET-MBP | MBP·tag |
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Citation: Chen J, Miao L, Li JM, Li YY, Zhu QY, Zhou CL, Fang HQ, Chen HP. Receptor-binding domain of SARS-Cov spike protein: Soluble expression in
E.coli , purification and functional characterization. World J Gastroenterol 2005; 11(39): 6159-6164 - URL: https://www.wjgnet.com/1007-9327/full/v11/i39/6159.htm
- DOI: https://dx.doi.org/10.3748/wjg.v11.i39.6159