Role of nuclear receptor CAR in carbon tetrachloride-induced hepatotoxicity

Figure 2 Histological changes associated with CCl₄ toxicity. (A) Histological findings in the liver; (a) CAR^–/– mice, control; (b) CAR^+/+ mice, control; (c) CAR^–/– mice, PB; (d) CAR^+/+ mice, PB; (e) CAR^–/– mice, CCl₄; (f) CAR^+/+ mice, CCl₄; (g) CAR^–/–mice, PB plus CCl₄; and (h) CAR^+/+ mice, PB plus CCl₄. Liver sections from each treatment were examined by hematoxylin and eosin staining. Liver samples from all treated animals were analyzed, but only representative histology is presented. The extent of the necrotic area in the CAR^+/+ liver was larger than that in CAR^–/– mice. PB pretreatment caused extensive centrilobular necrosis in CAR^+/+ mice. The arrows indicate centrilobular necrosis. Magnification, ×100. (B) The indices of centrilobular necrosis in CAR^+/+ mice and CAR^–/– mice. The indices of centrilobular necrosis were higher in CAR^+/+ mice than that in CAR^–/– mice with PB pretreatment. The index in CAR^+/+ mice treated with CCl₄ plus PB was significantly increased. The index of centrilobular necrosis was scored as follows: area of centrilobular necrosis divided by whole area. Data are mean±SD.