Focal adhesion kinase and Src phosphorylations in HGF-induced proliferation and invasion of human cholangiocarcinoma cell line, HuCCA-1

Figure 7 Treatment of cells with 0. 1, 0.5, and 1.0 µmol/L of AZM555130 for 48 h showed no inhibition of Src phosphorylation both in HGF-treated and untreated cells (A). The HGF-induced FAK phosphorylation was not inhibited after 48 h treatment of0.1 µmol/L AZM555130 but showed 34% and 63% inhibition by 0.5 and 1.0 µmol/L treatment, respectively. The 37% inhibition of FAK phosphorylation was observed by treatment of 1.0 µmol/L AZM555130 in non-induced cells (B). These values were compared with the levels of Src and FAK phosphorylation in AZM555130-untreated cells which is equal to 100%.