Focal adhesion kinase and Src phosphorylations in HGF-induced proliferation and invasion of human cholangiocarcinoma cell line, HuCCA-1

Figure 5 Treatment of HuCCA-1 cells with 20 ng/mL rhHGF for 0, 5, 15, 30, 60, and 120 min showed an increased degree of Src phosphorylation in a time-dependent manner. The maximal level of Src phosphorylation was reached at 30 min (A). Equal amount of Src protein (upper bands) used in each time point was confirmed by reprobing the membrane with anti-Src antibody (B). The density of bands was measured and pSrc/Src value was plotted in a graph (C).