Focal adhesion kinase and Src phosphorylations in HGF-induced proliferation and invasion of human cholangiocarcinoma cell line, HuCCA-1

Figure 4 Treatment of HuCCA-1 cells with 20 ng/mL rhHGF for 0, 5, 15, 30, 60, and 120 min showed increased degree of FAK phosphorylation in a time-dependent manner. The maximal level of FAK phosphorylation was reached at 60 min and then sharply decreased at 120 min (A). Equal amount of FAK protein used in each time point was confirmed by reprobing the membrane with anti-FAK antibody (B). The density of bands was measured and pFAK/FAK value was plotted in a graph (C).