Focal adhesion kinase and Src phosphorylations in HGF-induced proliferation and invasion of human cholangiocarcinoma cell line, HuCCA-1

Figure 2 mRNA expression level of HGF (A) and c-Met (B) was observed by RT-PCR technique. HuCCA-1 (CCA) expressed low HGF but a high level of c-Met mRNA. Liver myofibroblasts (MF) and hepatocellular carcinoma cell line (HepG2) were used as positive controls for HGF and c-Met, respectively. Equal amount of total RNA from each cell was confirmed by GAPDH (C).