Microarray analysis of Escherichia coli O157:H7

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Oct 7, 2005 (publication date) through Nov 4, 2024

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World Journal of Gastroenterology

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1007-9327

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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

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World J Gastroenterol. Oct 7, 2005; 11(37): 5811-5815
Published online Oct 7, 2005. doi: 10.3748/wjg.v11.i37.5811

Table 1 PCR primers and oligonucleotide probes for O157:H7 microarray

Targetgene	Primer sequences forPCR amplification	Labeledprimer	PCR productsize (bp)	Oligonucleotides targetadsorbed by array chip	GenBank No. ofBLAST search
O157	L5’AACGGTTGCTCTTCATTTAG3’	R	678	GGTGGAATGGTTGTCACGAATGACAAA	AE005429, AP002559, AB008676
(rfbE)	R5’GAGACCATCCAATAAGTGTG3’				AF061251 S83460
H7	L5’TACCACCAAATCTACTGCTG3’	R	560	GACGATGCAGGCAACTTGACGACTAA	AE005415, AF228496, U47614
(fliC)	R5’TACCACCTTTATCATCCACA3’				AP002559, AB028474, L07388
SLT1	L5’TGTAACTGGAAAGGTGGAGTATAC3’	R	210	GGATGACGTAACCATTAAAACTAATGCC	AY123842, AF461172,AJ251754,
	R5’GCTATTCTGAGTCAACGAAAAATAAC3’				AB071624, AE005442,X07903
SLT2	L5’TTTACGATAGACTTCTCGAC3’	L	228	AGATGGTCAAAACGCGCCTGATAGAC	AF329817, AE005296, AJ272135
	R5’CACATATAAATTATTTCGCTC3’				AB052227, AP002554, Y10775

Citation: Jin HY, Tao KH, Li YX, Li FQ, Li SQ. Microarray analysis of Escherichia coli O157:H7. World J Gastroenterol 2005; 11(37): 5811-5815
URL: https://www.wjgnet.com/1007-9327/full/v11/i37/5811.htm
DOI: https://dx.doi.org/10.3748/wjg.v11.i37.5811