Colorectal Cancer
Copyright ©The Author(s) 2005.
World J Gastroenterol. Sep 28, 2005; 11(36): 5644-5650
Published online Sep 28, 2005. doi: 10.3748/wjg.v11.i36.5644
Figure 2
Figure 2 Expression of PLK1 in frozen colon tissue and colon carcinoma cell lines. A: Expression of PLK1 protein (68 ku) in colon carcinoma and normal colon mucosa; B/C : Strong expression of PLK1 protein (B, immunoblot, 68 ku) and mRNA (C: RT-PCR, 1 807 bp) in colon carcinoma cell lines; D/E: Coexpression of PLK1 (red staining of the cytoplasm) and Ki-67 (brown staining of the nucleus) in RKO colon carcinoma cell line. A considerable number of cells were PLK1 positive, but remained Ki-67 negative (arrowheads). Note cells in M-phase (small arrows) strongly expressing both proteins. Original magnifications D: ×200, E: ×500.