Lysophosphatidic acid transactivates both c-Met and epidermal growth factor receptor, and induces cyclooxygenase-2 expression in human colon cancer LoVo cells

Figure 3 Effect of GM6001, an MMP inhibitor, on LPA-stimulated c-Met transactivation in colon cancer cells. Human colon cancer LoVo cells were serum-starved for 24 h and pre-incubated with the indicated concentrations of GM6001 for 30 min. After incubation with 10 µmol/L LPA for 2 min, cell lysates were obtained and IP with anti-c-Met. Immunoprecipitates were immunoblotted with anti-phosphotyrosine. Then the membrane was stripped and immunoblotted with anti-c -Met as a control. The same experiments were performed using anti-EGFR as a control for the inhibitory effect of GM6001.