Lysophosphatidic acid transactivates both c-Met and epidermal growth factor receptor, and induces cyclooxygenase-2 expression in human colon cancer LoVo cells

Figure 2 Effect of PTX, a Gi inhibitor, on LPA-stimulated c-Met transactivation in colon cancer cells. Human colon cancer LoVo cells were serum-starved for 24 h with or without 100 ng/mL PTX and then stimulated with 10 µmol/L LPA or 10 ng/mL HGF for 2 min. Cell lysates were IP with anti-c- Met. Immunoprecipitates were immunoblotted with anti-phosphotyrosine. Then the membrane was stripped and immunoblotted with anti-c -Met as a control. The same experiments were performed using anti-EGFR as a control for the inhibitory effect of PTX.