Lysophosphatidic acid transactivates both c-Met and epidermal growth factor receptor, and induces cyclooxygenase-2 expression in human colon cancer LoVo cells

Figure 1 Rapid tyrosine phosphorylation of c-Met in response to LPA in human colon cancer cells. (A) Human colon cancer LoVo cells were serum -starved for 24 h and then incubated with 10 µmol/L LPA for 2-60 min. After cell lysis, c -Met was immunoprecipitated (IP) using polyclonal anti-c -Met antibody, and immunoprecipitates were immunoblotted with monoclonal anti-phosphotyrosine antibody. Then the membrane was stripped and immunoblotted with anti-c-Met as a control. (B) Human colon cancer LoVo cells were serum-starved for 24 h and then incubated with 10 µmol/L LPA for 2-60 min. These cell lysates were IP with anti-phosphotyrosine, and the immunoprecipitates were immunoblotted with anti-c-Met.