Dendritic cells pulsed with hsp70-peptide complexes derived from human hepatocellular carcinoma induce specific anti-tumor immune responses

Figure 5 Maturation of human DCs by hsp70-peptide complexes derived from SMMC- 7721 cells. DCs were incubated with 20 μg/mL hsp70- peptide complexes derived from SMMC-7721 cells for 4 h at 37°C, and cultured for 48 h. The DCs were then harvested and analyzed for expression of the indicated surface molecules using FACS analysis. The percentages shown were positive for the indicated surface markers. The results are expressed as mean±SD and each value represents the mean of three replicates.