Dendritic cells pulsed with hsp70-peptide complexes derived from human hepatocellular carcinoma induce specific anti-tumor immune responses

Figure 2 Stimulation of T cells by autologous DCs pulsed with hsp70-peptide complexes derived from SMMC-7721 cells. DCs were incubated with 20 μg/mL hsp70-peptide complexes derived from SMMC- 7721 for 4 h at 37°C and cocultured with autologous T cells at different ratios for 5 d, and then tritiated thymidine was added and its incorporation was measured 18 h later. (A) 1 × 10⁵ T cells were cocultured with 2 × 10⁴ autologous DCs, pulsed with hsp70- peptide complexes (indicated as hsp70 stimulation) or unpulsed (indicated as DC control), or by themselves (indicated as T control) per well; (B) 1 × 10⁵ autologous T cells were cocultured with 2 × 10⁴, 1 × 10⁴, or 3.3 × 10³ DCs pulsed with hsp70-peptide complexes (indicated as hsp70 stimulation) or unpulsed (indicated as DC control) per well separately. The results are expressed as mean±SD and each value represents the mean of three replicates.