Dendritic cells pulsed with hsp70-peptide complexes derived from human hepatocellular carcinoma induce specific anti-tumor immune responses

Figure 1 Purification of hsp70-peptide complexes from HCC cell line SMMC-7721. (A) 12% reducing SDS-PAGE analysis of purified hsp70- peptide complexes preparation with silver staining. ( B) Western blotting analysis of purified hsp70-peptide complexes preparation. The preparation of hsp70-peptide complexes was immunoblotted with anti -hsp70 antibody. Lane 1 represents the protein molecular marker, and lane 2 represents hsp70-peptide complexes preparation.