Cancer-derived VEGF plays no role in malignant ascites formation in the mouse

Figure 1 Establishment of VEGF knockdown pancreatic cancer cell lines. A: Western blot showing marked reduction of VEGF expression in knockdown cells. Screening of the most effective targeting site for the VEGF gene. B: VEGF production of stable knockdown PancO2 cell clones. VEGF-WT and VEGF-KD clones (KD 1-10) were grown in 6-well plates, and the VEGF concentration in culture supernatant was measured using ELISA in triplicates. The growth medium was used as negative control (NC).