Study of transactivating effect of pre-S2 protein of hepatitis B virus and cloning of genes transactivated by pre-S2 protein with suppression subtractive hybridization

Figure 4 Reduction of G3PDH abundance by PCR-select subtraction. PCR was performed on unsubtracted (lanes1-4) or subtracted (lanes 5-8) secondary PCR products with the G3PDH 5’ and 3’ primers. Lanes 1, 5: 18 cycles; lanes 2, 6: 23 cycles; lanes 3, 7: 28 cycles; lanes 4, 8: 33 cycles. Lane M: DNA marker (2 000 bp).