Study of transactivating effect of pre-S2 protein of hepatitis B virus and cloning of genes transactivated by pre-S2 protein with suppression subtractive hybridization

Figure 2 RT-PCR products were eletrophoresed in 0. 9% agarose gel. Lane 1:negative control, lanes 2-4: mRNA was isolated from pcDNA3.1(-)-pre-S2 and RT-PCR was performed by three different Oligod T, lane 5: blank control, lane 6: positive control, M: DNA marker (2 000 bp).