Novel genetic variations of the p53R2 gene in patients with colorectal adenoma and controls

Figure 1 Structural feature and genetic variation analysis of p53R2. A: A representative p53R2 gene structure including a regulatory region with p53 binding sequence within intron 1; B: representative cold SSCP result. p53R2 Regulatory region was amplified, and PCR products were run on a 20% SSCP gel at 15 °C. Lanes 2 and 4 are the heterozygous polymorphism pattern; C: alterations in regulatory region of p53R2 gene: (1) DdeI G/C, and (2) DdeI C/C genotypes; D: Alterations in regulatory region of p53R2 gene: (1) NdeI G/G and (2) A/A genotypes; E: Alterations in regulatory region of p53R2 gene: (1) NdeI G/G and HhaI T/T, and (2) NdeI A/A and HhaI T/C genotypes; F: Alterations in regulatory region of p53R2 gene: 20 bp insertion which replaced an ATTTT between nt 1 831 and nt 1 835.