Novel rapid tissue lysis method to evaluate cancer proteins: Correlation between elevated Bcl-XL expression and colorectal cancer cell proliferation

Figure 4 Proteomic analysis of the human colorectal cancer tissue lysate prepared by the MagNA Lyser. Approximately 150 mg of tumor specimen from a colon cancer patient was subjected to tissue lysis by the new MagNA Lyser method. One aliquot (600 mg) of tissue lysate was resolved on a 13-cm IPG strip, pH 3-10, and subsequently run in a 14 cm×15 cm 12% SDS-polyacrylamide gel, then visualized by staining with Coomassie blue dye. Protein spots were cut from the gel, after trypsin digestion and peptide extraction, the spots were identified through MALDI-TOF mass spectrometry, and have been labeled on the gel image.