Novel rapid tissue lysis method to evaluate cancer proteins: Correlation between elevated Bcl-XL expression and colorectal cancer cell proliferation

Figure 1 Determination of the optimal specimen size from different organs for tissue lysis by the MagNA Lyser. Four specimen sizes, ranging from 100 to 600 mg, of rat organs were tested for the protein recovery rates from the new tissue lysis method using the MagNA Lyser. The oscillation frequency and duration was set at 6 500 r/min for 20, 40, 80, 120, or 180 s as indicated. Protein concentrations of tissue lysates were assayed by the Lowry method. The protein recovery rate from tissue lysis was calculated as the amount of total protein obtained divided by the original weight of the specimen. A: Liver; B: Stomach; C: Colon; D: Kidney; E: Spleen; F: Heart.