Trans-10,cis-12, not cis-9,trans-11, conjugated linoleic acid decreases ErbB3 expression in HT-29 human colon cancer cells

Figure 8 Effect of t10c12 on HRG-β-induced recruitment of the p85 subunit of PI3K to ErbB3 in HT-29 cells. Immunoprecipitation was performed with an anti-ErbB3 antibody and protein A-Sepharose as described above. The immunoprecipitated proteins were analyzed by Western blotting with an antibody against the p85 PI3K subunit. A: A photograph of chemiluminescent detection of the blot, which was representative of three independent experiments, is shown. B: The relative fold change in p85 to its own ErbB3 band on Western blots. Each bar represents the mean±SE (n = 3).