Trans-10,cis-12, not cis-9,trans-11, conjugated linoleic acid decreases ErbB3 expression in HT-29 human colon cancer cells

Figure 7 Effect of t10c12 on HRG-β-induced tyrosine phosphorylation of ErbB3 in HT-29 cells. Cells were treated in the absence or presence of 4 μmol/L t10c12 for 3 d and lysed without stimulation (0) or after 1, 5, or 60 min of stimulation with HRG-β. Cell lysates were incubated with an anti-ErbB3 antibody and the immune complexes were precipitated with protein A-Sepharose. The immunoprecipitated proteins were analyzed by Western blotting with antibodies against phosphotyrosine or ErbB3. A: Photographs of chemiluminescent detection of the blots, which were representative of three independent experiments, are shown; B: The relative abundance of each band was estimated by densitometric scanning of the exposed films. Each bar represents the mean±SE (n = 3).