Trans-10,cis-12, not cis-9,trans-11, conjugated linoleic acid decreases ErbB3 expression in HT-29 human colon cancer cells

Figure 6 Effect of c9t11 on ErbB1, ErbB2, and ErbB3 transcripts. HT-29 cells were treated with various concentrations of c9t11. Total RNA was isolated and RT-PCR was performed as described in Materials and methods. A: Photographs of the ethidium bromide-stained gel, which were representative of three independent experiments, are shown; B: Quantitative analysis of mRNAs. The relative abundance of each band was estimated by densitometric scanning of the exposed films. Each bar represents the mean±SE (n = 3).