Trans-10,cis-12, not cis-9,trans-11, conjugated linoleic acid decreases ErbB3 expression in HT-29 human colon cancer cells

Figure 3 Effect of t10c12 on the protein expression of ErbB1, ErbB2, ErbB3, HRG, and the p85 subunit of PI3K. HT-29 cells were treated with various concentrations of t10c12 for 3 d. Cell lysates were subjected to immunoblotting with an antibody against ErbB1, ErbB2, ErbB3, p85 subunit of PI3K, HRG, or β-actin. A: Photographs of chemiluminescent detection of the blots, which were representative of three independent experiments, are shown; B: Quantitative analysis of immunoblots. The relative abundance of each band was estimated by densitometric scanning of the exposed films. Each bar represents the mean±SE (n = 3). Comparisons between groups that yielded significant differences are indicated by different letters above each bar. For example, ^aP<0.05 vs 0 μmol/L t10c12, ^cP<0.05 vs 1 μmol/L t10c12, ^eP<0.05 vs 2 μmol/L t10c12.