Structural and functional aspects of the liver and liver sinusoidal cells in relation to colon carcinoma metastasis

Figure 2 CC531s were labeled with DiO, injected into the mesenteric vein. After 24 h, Kupffer cells (KC) were stained in vivo with TRITC-labeled latex beads, and the liver was fixed by perfusion. Confocal microscopy was conducted and images were named after corresponding laser lines. A: Group of CC531s was visualized; B: KC was visualized by the up-take of fluorescent latex beads; C: The rest of the field was examined with the transmission function; D: CC531s proliferating inside the liver parenchyma and inside the group of CC531s cells, a vessel was seen. The vessel is much straighter than the normal liver sinusoids, indicating a newly formed vessel. Image size: 158.7 μm×158.7 μm.