ACEI attenuates the progression of CCl4-induced rat hepatic fibrogenesis by inhibiting TGF-β1, PDGF-BB, NF-κB and MMP-2,9

Figure 4 Zymography analysis of activities of MMP-2 and MMP-9. Effect of perindopril on the activities of MMP-2 (72 ku) and MMP-9 (94 ku) in CCl4-induced hepatic fibrosis of rat. Liver samples were obtained as described. Protein (50 mg/lane) were separated by 7.5% gelatin containing SDS-PAGE, then remove SDS and incubate in zymogram incubation buffer as described. The clear band presents the activity of MMP-2,9. The activity of MMP-2,9 was elevated in Mo (4, 6 wk) compared with Nc and Pe (4, 6 wk).