Lewis blood genotypes of peptic ulcer and gastric cancer patients in Taiwan

Table 1 Primer sequences and restriction enzymes of mutation in Se and Le genes

Mutation	Primer sequence	Enzyme
Se gene
A385T	UP1: GATGGAGGAGGAATACCGCCTC	Ear I
	DP2: GATCTCCTGGCGGAGGTGGTGGTAGAAGATC
G428A	Identical to A358T primer pairs	Bgl II
C571T	UP: AGGAGATCCTCCAGGAGTTCA	Dde I
	DP: AGAAGGAGAAAAGGTCTCAAAGG
G849A	Identical to C571T primer pairs	Dde I
C628T	UP: AGTGTGGAAGGGGGTGGTGCCC	Bgl I
	DP: CCACTCTGGCAGGAAGGC
Fusion gene	UP: CTGCCTCCTGACCATGTCC	Pst I
	DP: identical to reverse primer of C628T
Le genes
T202C and C314T	UP: CCACCCTCCTGATCCTGCTC	Msp I (T202C)
	DP: GATATCCCAGTGGTGCACGATGATGATC	Bcl I (C314T)
C445A	UP: identical to UP of T202 C and C314 T	BstN I
	DP: GAGATTGAAGTATCTGTCCAAGGC
G508A	UP: TCAACTTGGAGCCACACCCT	Alu I
	DP: AGTTGGACACCGCCCAGGCCACCAG
A1007C	UP: GCTCCTTCCGCTGGGCACTAG	Alu I
	DP: TGGCCACAAAGGACTCCAGC
T1067A	UP: GTACCAGACGGTGCGATGCA	Nsi I
	DP: identical to DP of A1007 C

¹UP: upstream primer; ²DP: downstream primer; The mutated base is underlined.