Role of cell adhesion signal molecules in hepatocellular carcinoma cell apoptosis

Figure 1 Measurement of apoptosis by DNA fragmentation upon treatment with DCVC/DPPD. A: SMMC-7721 Hepatocellular carcinoma cells were treated with 0.1 mmol/LDCVC and 0.02 mmol/L DPPD for various time duration, then harvested for DNA fragmentation assay to estimate apoptosis. Lane 1, DNA marker; lane 2, absent DCVC; lanes 3-6 represent tumor cells treated for 1, 2, 4, 6, and 8 h, respectively; B: SMMC-7721 hepatocellular carcinoma cells were treated with 0.02 mmol/L DPPD and various DCVC concentrations for 6 h, then harvested for DNA fragmentation assay to estimate apoptosis. Lane 1, DNA marker; lane 2, absent DCVC; lanes 3–6 represent tumor cells treated for 0.02, 0.05, 0.1, and 0.2 mmol/L, respectively.