Biological impacts of “hot-spot” mutations of hepatitis B virus X proteins are genotype B and C differentiated

Table 2 Intracellular β-galactosidase activities of transfected Chang cells by pCMVβ and X gene expression vectors

DNA transfected	β-galactosidase activities1	P2	P3
pcDNA3.1/Hygro(–)	0.147 ± 0.039	-	-
XB	0.593 ± 0.051	< 0.01	-
XB-127	0.666 ± 0.054	< 0.01	< 0.05
XB-132	0.492 ± 0.052	< 0.01	< 0.01
XB-130-131	0.477 ± 0.065	< 0.01	< 0.01
XB-127-130-131	0.680 ± 0.090	< 0.01	< 0.05
XB-130-131-132	0.472 ± 0.081	< 0.01	< 0.05
XC	0.505 ± 0.064	< 0.01	-
XC-127	0.641 ± 0.044	< 0.01	< 0.01
XC-132	0.571 ± 0.104	< 0.01	> 0.05
XC-130-131	0.459 ± 0.065	< 0.01	> 0.05
XB-127-130-131	0.457 ± 0.067	< 0.01	> 0.05
XB-130-131-132	0.646 ± 0.177	< 0.01	> 0.05

¹All data are expressed as mean±SD of six separate experiments; ²Paired-samples t-test between pcDNA3.1/Hygro(–) and all HBx; ³Paired-samples t-test between genotype B(XB)/genotype C(XC) and the relative mutants.