Biological impacts of “hot-spot” mutations of hepatitis B virus X proteins are genotype B and C differentiated

Table 1 Sequences and related applications of the primers used for site-directed mutagenesis and nomenclature of HBx mutants

Primers	Sequences (5’→3’)	Applications		Nomenclature of the HBx mutants
		Nucleotide mutation2	Amino acid substitution3
R	CTCCTCCCCCAACTCTTCCCACTCAGTAAAC
F1	GAAGAGTTGGGGGAGGAGAC1	1753T→C	I127T	XB/XC-127
	TAGGTTAAAGGTC
F2	GAAGAGTTGGGGGAGGAGACTAGGTTA	1753T→C + 1762A→T + 1 764G→A	I127T + K130M + V131I	XB/XC-127-130-131
	ATGATCTTTGTACTAGG
F3	GAAGAGTTGGGGGAGGAGATTAGGTTA	1762A→T + 1764G→A	K130M + V131I	XB/XC-130-131
	ATGATCTTTGTACTAGG
F4	GAAGAGTTGGGGGAGGAGATTAGGTTA	1762A→T + 1764G→A + 1768T→A	K130M + V131I + F132Y	XB/XC-130-131-132
	ATGATCTATGTACTAGGAGGC
F5	GAAGAGTTGGGGGAGGAGATTAGGTTA	1768T→A	F132Y	XB/XC-132
	AAGGTCTATGTACTAGGAGGC

¹Mutated nucleotides are shown as underlined;

²1 753, 1 762, 1 764, 1 768 indicate the positions of mutated nucleotides in HBV genome;

³127, 130, 131, 132 are positions of substituted amino acids in HBx.