Temporal expression of hepatic inducible nitric oxide synthase in liver cirrhosis

Figure 2 Temporal iNOS mRNA expression in hepatocytes. At different time points after BDL operation, hepatocytes were isolated and iNOS mRNA expression was determined by competitive RT-PCR. A: Typical agarose gel of hepatic iNOS expression (representative of at least three separate experiments). B: Quantitative analysis of iNOS mRNA expression in hepatocytes in the process of cirrhosis development. 3d-4 wk, three days to four weeks after surgery. ^bP<0.01 for BDL versus sham-operated, 1-3 wk after surgery; ^dP<0.01 for 2 wk versus all other time points after BDL; ^fP<0.01 for 4 wk versus 3 wk after BDL.